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The safest approach is to follow the recommended dosage, typically 250500 mg per day

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Compound 2 , NADPH, and incubation time was varied as following: 1) TGR and 2 , no preincubation, 2) TGR, 2 , and NADPH, no preincubation, 3) TGR and 2 , 15 min preincubation, 4) TGR, 2 , and NADPH, 15 min preincubation, 5) TGR and NADPH followed by 15 min preincubation, 2 was added after 15 min preincubation, 6) TGR and 2 followed by 15 min preincubation, NADPH was added after 15 min preincubation