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Unlike the whole worm ChIP, the DNA amount after sequential ChIP was ultra low, so the NGS libraries were prepared directly with DNA on the streptavidin beads following manufacturers instructions (Kapa Biosystem, #KK8500)

doi:10.1016/j.ijcard.2014.03.145 Hoeck AD, Pall ML

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To obtain the necessary number of animals for the experiment, eight Wistar females were mated with eight Wistar males (1:1, individual cages) after checking the estrous cycle adapted from Marcondes and collaborators (2002)

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