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Isolated mRNA was then quantified with a NanoDrop spectrophotometer (Thermo Fisher Scientific, model ND-ONEC-W) and its quality measured in a 4150 TapeStation analyzer (Agilent, model G2992AA) using HSRNA ScreenTape (Agilent, catalog #5067-5579)

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Compounds that bear a quaternary or tertiary amine group (e.g