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Then the gene expression was quantified dependent on the LightCycler480 PCR system (Roche) by using 2SYBR Green qPCR Master Mix (biotool, #B21203)

However, numerous research indicate that immune system has a dual role in APAP-overdose challenge [36, 37, 39, 42, 43], which may be related to the specific roles of the immune cells in exacerbating liver damage or promoting liver repair and regeneration (Fig

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