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We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol

Manufactured by: Daedong, Korean

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After 72 h, RNA was extracted using an RNeasy Kit (Qiagen, QIA74106) according to the manufacturers instructions

During the first reaction, phosphopentose isomerase is involved in the conversion of ribose 5-phosphate into ribulose 5-phosphate

This material is sold for laboratory research use only