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When cells are exposed to oxidative and/or electrophilic stress, KEAP1 thiol residues are directly modified, which inactivates the ubiquitin E3 ligase activity of KEAP1-CUL3 complex, leading to stabilization of NRF2

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Samples were reconstituted in 3060 l 50% acetonitrile, followed by vortexing for 30 sec, and then centrifuged at 10,000 rpm for 10 min at 4 C

This isn't because 1:1 is mechanistically optimal in some provable way it's because researchers studying combinations of these compounds chose 1:1 as the default and the literature accumulated around that choice

So are many drugs used in endocrinology, reproductive medicine, and oncology including leuprolide (prostate cancer), oxytocin (labor induction), and vasopressin analogues (diabetes insipidus)