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At day 5 aggregates were gently collected, sieved through a reversible strainer and transferred into matrigel-coated plates with neural proliferation medium

Important: Do not skip titration steps

NF-B: nuclear factor-kappa B

[3] The risk of cardiovascular events was non-significantly reduced to 0.76 (95% confidence interval [CI] 0.46-1.28) and the risk of all-cause mortality was non-significantly reduced to 0.78 (95% CI 0.40-1.51)

For cell viability assays, medium was supplemented with 10% dialyzed FBS