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After 4 and 16 h exposure to exendin-4, significant degradation was noted in both cell types, as indicated by disappearance of the band corresponding to full-length SNAP-GLP-1R

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Although the K D value for KAR binding of ShHTL1 (77 M) was higher than the physiological concentration (1 M), this result might be because the fraction of active protein in the sample was lower than 100%, resulting in lower estimated affinity than real affinity