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Gradual dosing does not delay results

The following day, the cells were exposed to various concentrations of L P B-3 vesicles (0, 0.1, 0.2, 0.5 mg/mL) at 37 C under normoxic conditions (20% O 2 /5% CO 2 ) for 24 h

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Immunofluorescence staining and counting To stained c-fos, TNC tissues were collected 2 h after the last NTG injection, while for staining other targets, TNC was collected 24 h after the last NTG administration