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Pierce Streptavidin Coated High Capacity 96-well plates (Thermo Fisher Scientific, Cat #15500) was washed with TBST (1 Tris-buffered saline + 0.1% Tween 20) and subsequently coated with 100 L per well of 1 g/mL of biotinylated-drug in TBST + 0.1% BSA (bovine serum albumin) for 1 h at room temperature

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