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Thus, by contradiction, the survivors at high temperatures are not heat-tolerant mutants or persistor-like cells
Store lyophilized BPC-157 vials at -20C, away from light and moisture
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Ethical Approval Relevant ethics committees approved both study protocols
Plasmid construction To generate CRISPR-Cas9 plasmids for gene manipulation in AG-haESCs, specific sgRNAs were synthesized, annealed, and inserted into the BpiI (Thermo Fisher Scientific, Waltham, MA, USA)-linearized pX330-mCherry plasmid (Cat# 98750
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