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Seahorse oxymetry Cells were plated on XF96 culture plates (20,000 cells/well) in complete NB medium

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Time of day Twice-daily dosing (most common): Morning: 30-60 minutes before breakfast Evening: 30-60 minutes before dinner OR before bed Consistent timing each day helps Once-daily dosing: Morning preferred (on empty stomach) Or before bed for healing during sleep Choose what fits your schedule Does timing matter much

Syringes: Youll need at least two types

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10.1016/j.fitote.2020.104803 12 ChengX.LiuY.QiB.WangY.ZhengY.LiangX.et al (2024)