Materials and methods Cell culture and treatments Human 21NT breast cancer cells were cultured in 1 Modified Eagles medium alpha (alpha MEM) (Gibco), 2.8 M hydrocortisone, 1 g/ml insulin, 10% foetal calf serum (FCS), 1% gluta- max, 10 mM HEPES, 0.1 mM NEAA and 12.5 ng/ml Epithelial Growth Factor (EGF)
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Parenteral vs oral vitamin B12 in children with nutritional macrocytic anemia: a randomized controlled trial
PLoS ONE 5 , e8547 (2010)
For a lot of people, high-dose oral B12 raises levels about as well over a few months
Allow the vial to reach room temperature before introducing the solvent slowly against the inside wall and gently swirl until fully dissolved