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And mice were evaluated every 3 days for paw swelling scores and clinical scores, and the hind paw diameters were measured by a digital caliper

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This manifests as AKI, requiring renal replacement therapy in severe cases and significantly increasing mortality risk (97)

Subsequently, 25 L cell lysate or BSA standard was added to 200 L WR, followed by incubation for 30 min at 37C

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