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The intracellular metabolite extracts and 2 mL of spent culture medium containing extracellular metabolites were freeze-dried (BenchTop K manifold freeze dryer, VirTis) prior chemical derivatisation

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To test this hierarchy directly, we established stable Rptor-overexpressing HMC-3 cell lines (3.4-fold elevation at mRNA and protein levels) (Fig

Wong YC, Kim S, Peng W, Krainc D