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Sekhar RV, Patel SG, Guthikonda AP, et al

Approximately 95% of the oxygen consumed is reduced to water during aerobic metabolism, but the remaining fraction may be converted to reactive oxygen species and other free radicals, inducing oxidative stress

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In: Shils ME, Shike M, Ross AC, Caballero B, Cousins RJ, eds

The remaining steps were performed as described for the migration assay

The good news is that you can use glutathione in tandem with other antioxidants, so they boost each others stability and benefits