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These tend to be most noticeable at the start or after dose increases

Rockville, MD: United States Pharmacopeial Convention, 2020

After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample