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Genes Dev 14(2):163176 Gupta MK, Qin RY (2003) Mechanism and its regulation of tumor-induced angiogenesis

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It is always best to follow the instructions provided with the peptide for exact measurements

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After 48 hours of transfection, cell pellets were lysed gently in 250 l of lysis buffer [50 mM tris-HCl (pH 7.0), 0.5% NP-40, protease inhibitor cocktail (Roche Diagnostics), and 2.5% murine RNase inhibitor]

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