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This phosphorylation event subsequently activates various substrates, including insulin receptor substrate (IRS) and SRC homology 2 domain-containing protein (SHC), which in turn recruit additional signaling molecules to activate two major intracellular pathways: the phosphoinositide 3-kinase (PI3K)/serine-threonine kinase (AKT) pathway and the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway (Wang J