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[13] [19] Specifically, the function of GSTs in this role is twofold: to bind both the substrate at the enzyme's hydrophobic H -site and GSH at the adjacent, hydrophilic G-site, which together form the active site of the enzyme
Oxidized Glutathione (GSSG): This is the "spent" state
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Air and metals: oxygen and trace redox-active metals can speed up GSH oxidation
Immunogen: Thr34~Pro128