Vitamin B12 is water-soluble it quickly dissolves in water and does not remain in the body, being eliminated through urine within 2472 hours depending on physical activity and water intake
[113] Currently, radioisotope dilution assay (RIDA) with labeled vitamin B 12 and hog IF (pigs) have been used to determine vitamin B 12 content in food
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Diagnosis of B12 deficiency is complicated by the fact that levels of B12 in the blood are maintained even when stores are low at the expense of tissue levels