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For effective whitening results, regular doses are recommended for 4 to 12 weeks, followed by monthly maintenance sessions

Once the bacteria had been heat-shocked, they we plated on plates containing kanamycin, IPTG and x-gal

Most patients start noticing visible brightening within 24 weeks, with full results building over the course of treatment

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At 48 h post-transfection, cells were harvested in ice-cold PBS using Trypsin-EDTA (Lonza, Switzerland), centrifuged at 1000 g for 3 min, and washed with ice-cold PBS