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For tumours that are significantly LDH-dependent, as described above, genetic or pharmacological inhibition of LDHA effectively reduces cancer cell glycolytic flux and NADH/NAD ratio, and results in cancer cell apoptosis

Broad interest is now focused on the biguanide metformin 37, 38 (see review in the same issue of this topical issue) as well as IR and IGF-1R inhibitors

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Then transfer the cell suspension to the culture flask and incubate at conditions of 37 C under 5% CO until cells fully adhered