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In addition, the trial cohorts also had differences in baseline renal health, with the highest mean baseline eGFR in DECLARE TIMI-58

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Cells were washed twice with 10 ml 5% mannitol (Sigma-Aldrich), the mannitol was carefully and completely removed before 0.9 ml 100% methanol was added onto the cells, firmly rocking the dish so that the methanol covers the cell monolayer