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PLGA-based nanoparticles cross the BBB passively by adsorptive-mediated transcytosis (AMT), which is driven by electrostatic interactions between the endothelial surface of the BBB (negatively charged) and PLGA-based nanoparticles (positively charged)
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Our findings do not exclude a positive role for endosomal signaling or -arrestin-1 recruitment in acute responses to physiological GLP-1, but highlight how the kinetic context must be taken into account when exploiting noncanonical pathways during drug development
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It is important to understand that whilst Ozempic will not appear on routine drug screens, specialised laboratory tests can detect semaglutide if specifically requested
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