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Subjects were stratified by gender to achieve balance of baseline characteristics

For the brain fractionation as per The Thermo Scientific Pierce bicinchoninic acid Protein Assay Kit was used to quantify proteins in the tissue homogenates analyzed

Thus, cystine limitation suppressed mTORC1 and activated the ISR, the two major amino acid sensing pathways in HepG2 cells

Signs of degradation can include visible cloudiness, the presence of particulate matter, or a change in color in the solution