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Extracted proteins were quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific #23225)

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Measurements were obtained from the right lobe of the liver through the intercostal spaces, with a patient lying in the dorsal decubitus position and the right arm in maximal abduction

The structural modification of this peptide includes an arginine substitution at position 3 and an extended amino acid sequence, which influences receptor binding affinity and reduces susceptibility to proteolytic enzymes in controlled experimental conditions

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