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The RNAi construct was produced by amplifying a part of the adh-5 gene (exon 3) with the forward primer (5-ATAGGTACCCGTACCAGTTGAAATTCCAC-3) and the reverse primer (5-ATAGCGGCCGCGATTAACCGACACCCTTG-3)

Vohnout, B., de Gaetano, G., Donati, M

Diagnostic work-up and management of patients with isolated methylmalonic acidurias in European metabolic centres

In this specific cycle: 1 Hydrogen peroxide, a free radical and reactive oxygen species, is converted into water (harmless), and oxygen 2 Two molecules of reduced Glutathione [GSH (the active antioxidant form of glutathione)] are used by the Glutathione peroxidase enzyme to neutralize one molecule of hydrogen peroxide 3 NADPH is used by glutathione reductase enzyme to reduce ("recycle") glutathione disulfide back into usable reduced glutathione Key players: Selenium: Essential for the enzyme that initiates the breakdown of hydrogen peroxide Riboflavin (FAD - coenzyme form of riboflavin): essential for the enzyme that restores the oxidized Glutathione back to its more useful state

Journal of Pharmaceutical and Biomedical Analysis (2021) 4

Gut & Digestive Balance: Plant-based prebiotics help nourish the microbiome