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They were maintained in IMDM medium (Invitrogen), and DMEM medium (Invitrogen) correspondingly, supplemented with 10% fetal bovine serum (FBS) (S711-001S, Lonsera) and 1% Penicillin-Streptomycin (SV30010, HyClone)

Exposure to western diets pathologically alters the expression of many genes leading to atherosclerosis [16]

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First, we established a calibration protocol and membrane labeling strategy to localize membrane and proteins without errors associated with chromatic aberration or fluorescence polarization, allowing CSOP to maintain high resolution even with modest acoustic vibration (Fig