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For binding analyses, membranes were diluted in assay buffer (50 mM HEPES, 5 mM EGTA, 5 mM MgCl 2 , 0.005% Tween 20, pH = 7.4) to a final concentration of 0.2 mg/ml of protein and distributed (3 g/well) to 96-well OptiPlates
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Why should they be used carefully
At 28.5 billion CFUs upon manufacture (at least 15 billion CFUs upon expiration), our capsules contain 5 strains that were carefully selected for their potential to aid in weight loss, fat tissue mass reduction, and maintenance of intestinal health
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