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it's about protocol validity
It may help shift the production toward a lighter pigment (pheomelanin) rather than the darker one (eumelanin)
As an antioxidant, glutathione is great at fighting off harmful elements in our skin, which helps reduce dark spots and evens out skin tone
A 10 g cell pellet was resuspended in 30 ml buffer B (25 mM Tris-HCl, pH 7.5, 10% glycerol, 0.01% Igepal CA-630, 2 mM 2-Mercaptoethanol) containing 500 mM KCl and a cocktail of protease inhibitors (PIs) (5 g/ml each of aprotinin, chymostatin, leupeptin, and pepstatin A) and lysed by sonication (QSonica Q700) at an amplitude of 40 for two min with 20 s pulses
Those are not the same, please clarify GM Amber