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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Future Perspectives Certain lipid metabolic pathways directly influence neuronal maturation and differentiation (Knobloch and Jessberger 2017)

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Medications : Acetaminophen (Tylenol) is notorious for depleting glutathione

Hepatic APAP metabolism results in the production of the reactive metabolite N -acetyl- p -benzoquinone imine (NAPQI), which under physiological conditions is cleared by its conjugation with glutathione (GSH) to prevent its targeting to mitochondria