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Buffers The 2X Radioimmunoprecipitation Assay Buffer, commonly known as RIPA, consists of 100 mM Tris (tris(hydroxymethyl)aminomethane) titrated with HCl to pH 7.4, 300 mM NaCl, 2 mM ethylenediaminetetraacetic acid, 2 mM NaF, 2 mM Na 3 VO 4 , 2% NP-40, and 0.5% sodium deoxycholate

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Amino acid excipients like histidine and methionine can act as antioxidant buffers, sacrificing themselves to oxidation before the active peptide is affected