Isolated hepatocytes were plated on collagen-coated 6-well plates (4 10 4 cells per cm 2 ) in DMEM (Gibco #12800-017, containing 1 mM pyruvate, 25 mM glucose, and 4 mM glutamine) supplemented with 10 % (vol/vol) fetal bovine serum (FBS), 100 units/ml penicillin, 100 g/ml streptomycin, 1 M dexamethasone, 0.1 M insulin, and an additional 1 mM sodium pyruvate and incubated at 37 C in an atmosphere of 5 % CO 2
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L-Carnitine is an essential nutrient for vegetarians and those following strict dietary regimens, as the precursor amino acids and other necessary components for its synthesis may be insufficient or lacking in their diet
Related papers Life Sciences, 1992 ~,mrnRry The major low molecular weight thiol inside cells, the tripeptide glutathione (GSH), is of importance for protection of the cell against oxidative challenge, for thiol homeostasis required to guarantee basic functions, and for defence mechanisms against xenobiotics
Meals can feel less experimental, and food decisions may take less mental effort
had an additional history of STEMI + percutaneous coronary intervention (PCI) or non-STEMI
