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However, previous studies have not resolved certain questions: i) whether alterations in the identified proteins and metabolites are affected by eye drops, ii) how these changes in proteins and metabolites are affected by glaucoma phenotype, and iii) whether normal-tension glaucoma shows protein and metabolite changes in the same way as POAG

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Given the peptide molecular weight of 5,414.9 Da and 39 amino acid sequence including non-standard residues, mass spectrometry confirmation of the correct molecular species is essential