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After a further centrifugation (200 x g, 8 min, 20C, Universal 320, Andreas Hettich GmbH, Tuttlingen, Germany), PBMC were resuspended in RPMI medium supplemented with fetal bovine serum, HEPES buffer, L-glutamine, and -mercaptoethanol (all purchased from Biochrom AG, Berlin, Germany)
While prevalence data for subclinical gut overgrowth is difficult to quantify precisely because it is underdiagnosed functional medicine practitioners report that it is among the most common root-cause findings in patients presenting with chronic fatigue, irritable bowel syndrome, and treatment-resistant mood disorders
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J Biol Chem 278:1099311001 Blandizzi C, Natale G, Gherardi G, Lazzeri G, Marveggio MC, Colucci R, Carignani D, Del Tacca M (1999) Acid-independent gastroprotective effects of lansoprazole in experimental mucosal injury
The Immune Defense Supplements collection is a natural next stop if you want to explore more support alongside glutathione
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