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Delivery method is another major factor

In the context of surgery, prophylactic DNase might reduce postoperative SIRS (though could have potential side effect of impairing host defense against infection, so careful balance needed)

After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h

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