For immunofluorescence, cryosections were dried for 2 h at RT, fixed in pure acetone for 10 min and blocked with PBS 10% FCS for 30 min at RT
Storage, Disposal, and When to Seek Medical Advice Follow your trial protocol for storage
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Effective transdermal delivery would require advanced technologies such as: Microneedle arrays : Tiny needles that create microscopic channels in the skin to facilitate drug absorption (these are minimally invasive devices rather than traditional patches) Chemical penetration enhancers : Substances that temporarily increase skin permeability Iontophoresis : Using electrical currents to drive charged molecules through the skin Nanoparticle formulations : Encapsulating drugs in carriers designed to cross skin barriers Several preclinical and early-stage studies have explored these approaches for GLP-1 delivery, with some promising results in laboratory and animal models
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RECAP was the only study to explore order of initiation, showing no significant difference in kidney outcomes based on whether GLP-1 RA or SGLT2i therapy was initiated first [17]
