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Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template
November 1, 2022
In the study, researchers added cysteine and glycine to the subjects diets, and they began to notice increased glutathione levels after two weeks
This unique iron dependency renders tumor cells to become susceptible to iron overload, making it possible to develop ferroptosis-mediated therapy
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Urinalysis : Urinary ketones 2+ have similar implications to an elevated level of beta-hydroxybutyrate (below)
