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Human embryonic kidney 293T cells were treated with LipoDuo or plain glutathione to assess intracellular uptake, optimal dosing, cell proliferation and wound healing

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Western blotting Samples were lysed in RIPA buffer containing a protease inhibitor, and the total protein content was quantified using a bicinchoninic acid protein assay kit (Beyotime)

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This allows distinction between short term effect under up titration and more sustained impacts of GLP-1 RA, DPP-4 inhibitors or SGLT-2 inhibitors