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Purified membranes were resuspended in 40 mL low salt buffer and incubated with 100 M PF-06372222, 2 mg mL 1 iodoacetamide, and EDTA-free protease inhibitor cocktail tablet for 1 h at 4 C

CrossRef Ubiquitin-Specific Protease 2 (USP2) as a Modulator of Energy Metabolism: A Review of Studies Using Animal and Cellular Models Hiroshi Kitamura, Jun Okabe, Himeka Hayashi, Tomohito Iwasaki Biomedicines .2026