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E1 is a thiamin pyrophosphate (TPP)-dependent -ketoacid dehydrogenase, R -lipoic acid functions as a prosthetic group essential for E2 transacetylase activity, and E3 is a flavin adenine dinucleotide (FAD)-dependent dihydrolipoamide dehydrogenase ( Figure 4 )

doi: 10.1002/chem.201704561 173 CzerneckiT
This variability is normal and does not reflect personal failure when your timeline differs from others or from published averages

Approximately 45 min before running the assay, the distilled water in the probe plate was replaced with 200 l XF calibrant and incubated at 37 C, no CO 2

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