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RNA, DNA isolation and PCR RNA was isolated using NucleoSpin RNA Kit (Macherey-Nagel 740955250)

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Gather your supplies : your GLP-1 pen, a new needle (if using a pen with replaceable needles), and an alcohol wipe or cotton wool with surgical spirit

Physiology and pharmacology of effects of GLP-1-based therapies on gastric, biliary and intestinal motility

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the weighted Charlson Comorbidity Index (CCI) which have been shown to improve the performance over the original score with c-statistics of 0.8 61,62