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Shaking creates foam and exposes peptide molecules to air-liquid interface stress, which accelerates oxidation and aggregation

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GLP-1 expression was downregulated in wild-type Lox5 cells knocked out by FNDC5, and the induced expression of lipolysis and autophagy-related genes and proteins were also inhibited, such as adipose triacylglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and hepatic lipase deficiency gene (LIPC)

The randomization was scheduled using SAS programming code