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For the western blotting of HMGB1, frozen cortices were homogenized in 50 mM TrisHCl (pH 7.5) containing protease inhibitor cocktail and phosphatase inhibitor cocktail

HMSC-E4 promoted enhanced cartilage formation, as evidenced by intensified Safranin-O and type II collagen (COL2) staining, higher Bern scores, and increased expression of type X collagen ( COL10A1 ) and COL1A1