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Then cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kits (4374967, Applied Biosystems) with the accompanied random primers, as described previously ( The mRNA expression levels of given genes in the transfected cells with or without cell sorting enrichment were determined by qPCR using Taq388 mix (Supplementary Table S3

While all cell types were affected, senescence was most pronounced in the acinar cells and fibroblasts

There can also be long-term implications

To maintain the benefits over time, you'll need maintenance sessions and healthy lifestyle choices

Al-Arafat, T., Mao, A., Katsube, T., & Wang, B

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