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Lyons J, Rauh-Pfeiffer A, Yu YM, Lu XM, Zurakowski D, Tompkins RG, Ajami AM, Young VR, Castillo L: Blood glutathione synthesis rates in healthy adults receiving a sulfur amino acid-free diet

Front Pharmacol 7:

Oxidative stress-induced formation of methionine sulfoxide can spontaneously react with hydroxyl radical and generates methyl radical that non-specifically and non-enzymatically methylate DNA cytosine and may affect the epigenome 38

HPLC alone cannot confirm peptide identity mass spectrometry is required to verify that the measured compound is actually the target peptide and not a co-eluting impurity with similar retention characteristics

It causes oxidative stress