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PowerPoint slides Rights and permissions About this article Cite this article Picardo, M., Dell'Anna, M., Ezzedine, K

The GAPDH substrate 1,3 bisphosphoglycerate was generated by incubating 13.5 mM 3-phosphoglycerate, 6 mM ATP with 0.012 U/l 3-phosphoglycerate phosphokinase in 100 mM Tris/HCl p H 8.0, 1 mM EDTA, 15 mM MgCl 2 for 20 to 30 min at RT