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Cells were sub-cultured after treatment with 0.25% TrypsinEDTA once they reached coverage of 90%

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Although MICs are higher than those of standard azoles or terbinafine in vitro, the use of topical formulations at percent-level concentrations makes these MICs clinically relevant

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Diagnosis is largely clinical , supported by biomarkers , symptom history , and ruling out other conditions (like systemic mastocytosis or allergies)

Furthermore, we developed a highly selective Smurf1 degrader, Smurf1-antagonizing repressor of tumor 1, which exhibits efficient PDK1Akt blockade and potent tumor suppression alone or combined with PDK1 inhibitor in KRAS-mutated CRC