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Gut organ culture system and X-IPA permeability assay Fabrication of the gut organ culture device and gut organ culture experiments (Yissachar et al

Remove from heat and let stand for 20 minutes to allow the broth to absorb nutrients and flavors

Those answers matter more than a chart copied from the internet

The assay revealed however also a higher potency for SFN than for AITC, which might be due to differences in physicochemical properties such as their ability to cross the cell membrane, compound stability or differences in reactivity